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Description
| Analysis Note | Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pHÂ 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C. |
| Biochem/physiol Actions | Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6. |
| Preparation Note | Con A (C 7275) adsorbed to colloidal gold; mean particle size 17-23 nm; monodisperse. |
| Physical form | Suspension in approx. 50% glycerol containing 0.15 M NaCl, 0.01 M sodium phosphate, pH 6.8, 0.1 mM Ca2+, 0.1 mM Mn2+, 0.02% PEG 20 and 0.02% sodium azide |
Properties
| form | aqueous glycerol suspension |
| concentration | ~5 A520 units/mL |
| total impurities | Carbohydrate, essentially free |
| nominal | 20 nm (colloidal gold) |
| conjugate | gold conjugate |
| storage temp. | −20°C |
References
| reference | Benhamou, N. and Ouellette, G.B. J. Histochem. Cytochem. 34, 855, (1986) Abstract |
| | Reeke, G.N., et al. Ann. N.Y. Acad. Sci. 234, 369, (1974) |
| | Kalb, A.J. and Lustig, A. Biochim. Biophys. Acta 168, 366, (1968) |
| | Gunther, G.R., et al. Proc. Natl. Acad. Sci. U. S. A. 70, 1012, (1973) |
| | , Bittiges, H. and Schnebli, H.P, ed. Concanavalin A as a Tool London , (1976) |
| Merck | Merck 13,2521 |
